Bicuculline increases anxiety-like behavior in PKCε-null mice. Data are from 25 wild-type and 23 PKCε-null mice treated with saline and 20 wild-type and 19 PKCε-null mice treated with bicuculline. Two-factor ANOVA revealed main effects of treatment (F1,84 = 4.2, P = 0.043) on the percentage of total entries into the open arms (a) and genotype (F1,84 = 4.4, P = 0.039) on the percentage of time spent in the open arms (b). There were no significant effects of either factor on the number of closed-arm entries (c). *P < 0.05 compared with saline-treated wild-type mice, and P < 0.05 compared with saline-treated PKCε-null mice (Tukey’s test).