Determination of the molecular species composition of diacylglycerols in human adipose tissue by solid-phase extraction and gas chromatography on a polar phase
YM Pacheco, MC Pérez-Camino, A Cert… - … of Chromatography B …, 1998 - Elsevier
The free diacylglycerols (DAGs) in adipose tissue are involved in the metabolism of stored
lipids and hence are related to the supply of fatty acids for other tissues. This paper
describes a simple, fast, and reproducible method for the identification and quantification of
different molecular species of DAGs in human adipose tissue. The method comprised solid-
phase extraction on a diol-bonded phase column combined with capillary GC analysis of
silylated DAG derivatives on a polar phase (65% phenylmethylsilicone). Separation of the …
lipids and hence are related to the supply of fatty acids for other tissues. This paper
describes a simple, fast, and reproducible method for the identification and quantification of
different molecular species of DAGs in human adipose tissue. The method comprised solid-
phase extraction on a diol-bonded phase column combined with capillary GC analysis of
silylated DAG derivatives on a polar phase (65% phenylmethylsilicone). Separation of the …