Two subpopulations of human T lymphocytes expressing different antigen receptors, α / β and γ / δ, emigrate into inflamed tissues in distinctive patterns. We compared the transmigration of α / β and γ / δ T cells to C‐C and C‐X‐C chemokines using an in vitro transendothelial chemotaxis assay. The C‐C chemokines monocyte chemoattractant protein (MCP)‐1, RANTES, macrophage inflammatory protein (MIP)‐1α and MIP‐1β stimulated similar, dose‐dependent chemotaxis of purified γ / δ T cells, whereas MCP‐1, RANTES, and MIP‐1α pro duced greater chemotaxis of purified α / β T cells than MIP‐1β. In contrast, the C‐X‐C chemokines interleukin (IL)‐8 and interferon‐γ inducible protein‐10 (IP‐10) did not promote chemotaxis of either α / β or γ / δ T cells. Three γ / δ T cell clones with differing CD4 and CD8 phenotypes also migrated exclusively to C‐C chemokines. Phenotypic analysis of mononuclear cells that transmigrated from an input population of unfractionated peripheral blood mono nuclear cells confirmed the results with purified γ / δ T cells. Our data demonstrate that human peripheral blood α / β and γ / δ T cells can transmigrate to MCP‐1, RANTES, MIP‐1α, and MIP‐1β, and suggest that both T lymphocyte subpopulations share the capacity to emigrate in response to C‐C chemokines during inflammation.