Despite few published studies that assess the accuracy of glucometers in laboratory animals, glucometers are commonly used in animal research. We set out to determine the accuracy of 5 point-of-care glucometers (POCG) when used to evaluate murine whole blood, plasma, and serum samples. The POCG tested included one veterinary device (POCG A) and 4 humanuse instruments (POCG B through E). Whole blood, plasma, and serum samples from 50 female C57BL/6J mice were analyzed on all POCG, and serum was analyzed on a reference biochemical analyzer. The mean blood glucose concentration (BGC) measured in whole blood by using POCG A was greater than that on the biochemical analyzer, whereas the mean BGC in whole blood according to POCG B through E did not differ significantly from that on the biochemical analyzer. Mean BGC in plasma and serum did not differ between POCG B and E and the biochemical analyzer, whereas the plasma and serum BGC values from POCG C and D were greater than the mean BGC from the biochemical analyzer. The accuracy of each POCG for each sample type was evaluated by analyzing mean differences, correlations, and Bland–Altman graphs. We found that the 4 human-use POCG are appropriate for use with whole blood from female C57BL/6J mice, whereas only 2 of the evaluated POCG were sufficiently accurate for use with plasma or serum.