Importance The sensitivity of dried blood spots (DBS) to identify newborns with congenital
cytomegalovirus (cCMV) infection has not been evaluated in screening studies using the
current, higher-sensitivity methods for DBS processing. Objective To assess the sensitivity of
DBS polymerase chain reaction (PCR) for newborn screening for cCMV infection using
saliva as the reference standard for screening, followed by collection of a urine sample for
confirmation of congenital infection. Design, Setting, and Participants This population-based …