[HTML][HTML] Functional redundancy of steroid C26-monooxygenase activity in Mycobacterium tuberculosis revealed by biochemical and genetic analyses
JB Johnston, H Ouellet, PRO de Montellano - Journal of biological …, 2010 - ASBMB
One challenge to the development of new antitubercular drugs is the existence of multiple
virulent strains that differ genetically. We and others have recently demonstrated that
CYP125A1 is a steroid C 26-monooxygenase that plays a key role in cholesterol catabolism
in Mycobacterium tuberculosis CDC1551 but, unexpectedly, not in the M. tuberculosis
H37Rv strain. This discrepancy suggests that the H37Rv strain possesses compensatory
activities. Here, we examined the roles in cholesterol metabolism of two other cytochrome …
virulent strains that differ genetically. We and others have recently demonstrated that
CYP125A1 is a steroid C 26-monooxygenase that plays a key role in cholesterol catabolism
in Mycobacterium tuberculosis CDC1551 but, unexpectedly, not in the M. tuberculosis
H37Rv strain. This discrepancy suggests that the H37Rv strain possesses compensatory
activities. Here, we examined the roles in cholesterol metabolism of two other cytochrome …