Molecular cloning of the rat inducible nitric oxide synthase gene promoter
W Eberhardt, D Kunz, R Hummel… - … and biophysical research …, 1996 - Elsevier
W Eberhardt, D Kunz, R Hummel, J Pfeilschifter
Biochemical and biophysical research communications, 1996•ElsevierThe inducible nitric oxide synthase (iNOS) is not constitutively expressed but is induced in
many types of mammalian cells by cytokines and bacterial endotoxins. Previously, we have
reported the most interesting feature of rat iNOS gene that is up-regulated by cyclic AMP at
transcriptional level as demonstrated by nuclear run-on assay. We now have isolated and
sequenced the rat iNOS gene promoter and determined the transcription start site.
Moreover, we have constructed a rat iNOS promoter-chloramphenicol acetyltransferase …
many types of mammalian cells by cytokines and bacterial endotoxins. Previously, we have
reported the most interesting feature of rat iNOS gene that is up-regulated by cyclic AMP at
transcriptional level as demonstrated by nuclear run-on assay. We now have isolated and
sequenced the rat iNOS gene promoter and determined the transcription start site.
Moreover, we have constructed a rat iNOS promoter-chloramphenicol acetyltransferase …
The inducible nitric oxide synthase (iNOS) is not constitutively expressed but is induced in many types of mammalian cells by cytokines and bacterial endotoxins. Previously, we have reported the most interesting feature of rat iNOS gene that is up-regulated by cyclic AMP at transcriptional level as demonstrated by nuclear run-on assay. We now have isolated and sequenced the rat iNOS gene promoter and determined the transcription start site. Moreover, we have constructed a rat iNOS promoter-chloramphenicol acetyltransferase (CAT) fusion gene and analyzed its inducibility by interleukin 1β and cyclic AMP in transiently transfected Swiss 3T3 fibroblasts.
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