Luminal prostaglandin E2 modulates sodium and water transport in rabbit cortical collecting ducts

Y Ando, Y Asano - American Journal of Physiology-Renal …, 1995 - journals.physiology.org
Y Ando, Y Asano
American Journal of Physiology-Renal Physiology, 1995journals.physiology.org
We have previously found that arginine vasopressin (AVP) acts not only from the basolateral
side but also from the luminal side of the rabbit cortical collecting duct (CCD). In the present
study, we examined whether prostaglandin E2 (PGE2), another classic and potent
modulator of the collecting duct functions, exerts luminal actions in the rabbit CCD perfused
in vitro. Although luminal prostaglandin I2 was inert, luminal PGE2 (> 1 nM) induced
transient hyperpolarization of transepithelial voltage followed by sustained depolarization in …
We have previously found that arginine vasopressin (AVP) acts not only from the basolateral side but also from the luminal side of the rabbit cortical collecting duct (CCD). In the present study, we examined whether prostaglandin E2 (PGE2), another classic and potent modulator of the collecting duct functions, exerts luminal actions in the rabbit CCD perfused in vitro. Although luminal prostaglandin I2 was inert, luminal PGE2 (> 1 nM) induced transient hyperpolarization of transepithelial voltage followed by sustained depolarization in a dose-dependent manner. This action was preserved in the presence of basolateral PGE2, luminal AVP, or luminal BaCl2, but abolished by basolateral ouabain or luminal amiloride. Furthermore, unlike luminal AVP, luminal PGE2 suppressed Na transport and increased osmotic water permeability. The present study suggests that PGE2, similar to AVP but in a different fashion, modulates transepithelial transports from both luminal and basolateral sites in the CCD in vivo.
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