[HTML][HTML] miR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program

S Su, Q Zhao, C He, D Huang, J Liu, F Chen… - Nature …, 2015 - nature.com
S Su, Q Zhao, C He, D Huang, J Liu, F Chen, J Chen, JY Liao, X Cui, Y Zeng, H Yao, F Su…
Nature communications, 2015nature.com
Macrophages play a pivotal role in tissue fibrogenesis, which underlies the pathogenesis of
many end-stage chronic inflammatory diseases. MicroRNAs are key regulators of immune
cell functions, but their roles in macrophage's fibrogenesis have not been characterized.
Here we show that IL-4 and IL-13 induce miR-142-5p and downregulate miR-130a-3p in
macrophages; these changes sustain the profibrogenic effect of macrophages. In vitro, miR-
142-5p mimic prolongs STAT6 phosphorylation by targeting its negative regulator, SOCS1 …
Abstract
Macrophages play a pivotal role in tissue fibrogenesis, which underlies the pathogenesis of many end-stage chronic inflammatory diseases. MicroRNAs are key regulators of immune cell functions, but their roles in macrophage’s fibrogenesis have not been characterized. Here we show that IL-4 and IL-13 induce miR-142-5p and downregulate miR-130a-3p in macrophages; these changes sustain the profibrogenic effect of macrophages. In vitro, miR-142-5p mimic prolongs STAT6 phosphorylation by targeting its negative regulator, SOCS1. Blocking miR-130a relieves its inhibition of PPARγ, which coordinates STAT6 signalling. In vivo, inhibiting miR-142-5p and increasing miR-130a-3p expression with locked nucleic acid-modified oligonucleotides inhibits CCL4-induced liver fibrosis and bleomycin-induced lung fibrosis in mice. Furthermore, macrophages from the tissue samples of patients with liver cirrhosis and idiopathic pulmonary fibrosis display increased miR-142-5p and decreased miR-130a-3p expression. Therefore, miR-142-5p and miR-130a-3p regulate macrophage profibrogenic gene expression in chronic inflammation.
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