Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis
MD Hulett, C Freeman, BJ Hamdorf, RT Baker… - Nature medicine, 1999 - nature.com
MD Hulett, C Freeman, BJ Hamdorf, RT Baker, MJ Harris, CR Parish
Nature medicine, 1999•nature.comThe endoglycosidase heparanase is an important in the degradation of the extracellular
matrix by invading cells, notably metastatic tumor cells and migrating leukocytes. Here we
report the cDNA sequence of the human platelet enzyme, which encodes a unique protein of
543 amino acids, and the identification of highly homologous sequences in activated mouse
T cells and in a highly metastatic rat adenocarcinoma. Furthermore, the expression of
heparanase mRNA in rat tumor cells correlates with their metastatic potential. Exhaustive …
matrix by invading cells, notably metastatic tumor cells and migrating leukocytes. Here we
report the cDNA sequence of the human platelet enzyme, which encodes a unique protein of
543 amino acids, and the identification of highly homologous sequences in activated mouse
T cells and in a highly metastatic rat adenocarcinoma. Furthermore, the expression of
heparanase mRNA in rat tumor cells correlates with their metastatic potential. Exhaustive …
Abstract
The endoglycosidase heparanase is an important in the degradation of the extracellular matrix by invading cells, notably metastatic tumor cells and migrating leukocytes. Here we report the cDNA sequence of the human platelet enzyme, which encodes a unique protein of 543 amino acids, and the identification of highly homologous sequences in activated mouse T cells and in a highly metastatic rat adenocarcinoma. Furthermore, the expression of heparanase mRNA in rat tumor cells correlates with their metastatic potential. Exhaustive studies have shown only one heparanase sequence, consistent with the idea that this enzyme is the dominant endoglucuronidase in mammalian tissues.
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