[HTML][HTML] Ceramide regulates atypical PKCζ/λ-mediated cell polarity in primitive ectoderm cells: a novel function of sphingolipids in morphogenesis
In mammals, the primitive ectoderm is an epithelium of polarized cells that differentiates into
all embryonic tissues. Our study shows that in primitive ectoderm cells, the sphingolipid
ceramide was elevated and co-distributed with the small GTPase Cdc42 and cortical F-actin
at the apicolateral cell membrane. Pharmacological or RNA interference-mediated inhibition
of ceramide biosynthesis enhanced apoptosis and impaired primitive ectoderm formation in
embryoid bodies differentiated from mouse embryonic stem cells. Primitive ectoderm …
all embryonic tissues. Our study shows that in primitive ectoderm cells, the sphingolipid
ceramide was elevated and co-distributed with the small GTPase Cdc42 and cortical F-actin
at the apicolateral cell membrane. Pharmacological or RNA interference-mediated inhibition
of ceramide biosynthesis enhanced apoptosis and impaired primitive ectoderm formation in
embryoid bodies differentiated from mouse embryonic stem cells. Primitive ectoderm …
