Activation of PI3-kinase stimulates endocytosis of ROMK via Akt1/SGK1-dependent phosphorylation of WNK1
CJ Cheng, CL Huang - Journal of the American Society of …, 2011 - journals.lww.com
WNK kinases stimulate endocytosis of ROMK channels to regulate renal K+ handling.
Phosphatidylinositol 3-kinase (PI3K)-activating hormones, such as insulin and IGF 1,
phosphorylate WNK1, but how this affects the regulation of ROMK abundance is unknown.
Here, serum starvation of ROMK-transfected HEK cells led to an increase of ROMK current
density; subsequent addition of insulin or IGF1 inhibited ROMK currents in a PI3K-
dependent manner. Serum and insulin also increased phosphorylation of the downstream …
Phosphatidylinositol 3-kinase (PI3K)-activating hormones, such as insulin and IGF 1,
phosphorylate WNK1, but how this affects the regulation of ROMK abundance is unknown.
Here, serum starvation of ROMK-transfected HEK cells led to an increase of ROMK current
density; subsequent addition of insulin or IGF1 inhibited ROMK currents in a PI3K-
dependent manner. Serum and insulin also increased phosphorylation of the downstream …