High frequency of skewed X-chromosome inactivation in females with autoimmune thyroid disease: a possible explanation for the female predisposition to thyroid …
TH Brix, GPS Knudsen, M Kristiansen… - The Journal of …, 2005 - academic.oup.com
TH Brix, GPS Knudsen, M Kristiansen, KO Kyvik, KH Ørstavik, L Hegedüs
The Journal of Clinical Endocrinology & Metabolism, 2005•academic.oup.comContext: Autoimmune thyroid diseases (AITD) comprise Graves' disease (GD) and
Hashimoto's thyroiditis (HT). They are characterized by loss of immunological self-tolerance
and female preponderance. Theoretically, X chromosome inactivation (XCI) and resultant
tissue chimerism could offer an explanation for the female predisposition to AITD. Aim: Our
aim was to examine whether skewed XCI is associated with AITD. Designs: We first
conducted a classical case-control study of twin individuals with and without AITD, and then …
Hashimoto's thyroiditis (HT). They are characterized by loss of immunological self-tolerance
and female preponderance. Theoretically, X chromosome inactivation (XCI) and resultant
tissue chimerism could offer an explanation for the female predisposition to AITD. Aim: Our
aim was to examine whether skewed XCI is associated with AITD. Designs: We first
conducted a classical case-control study of twin individuals with and without AITD, and then …
Abstract
Context: Autoimmune thyroid diseases (AITD) comprise Graves’ disease (GD) and Hashimoto’s thyroiditis (HT). They are characterized by loss of immunological self-tolerance and female preponderance. Theoretically, X chromosome inactivation (XCI) and resultant tissue chimerism could offer an explanation for the female predisposition to AITD.
Aim: Our aim was to examine whether skewed XCI is associated with AITD.
Designs: We first conducted a classical case-control study of twin individuals with and without AITD, and then a case-control study of twin pairs discordant for AITD.
Participants: Participants included 32 female twins with AITD and a control group of 96 healthy female twin individuals.
Methods: XCI analysis was performed by enzymatic predigestion of DNA with a methylation-sensitive enzyme followed by PCR of the polymorphic CAG repeat of the androgen receptor gene. The XCI pattern was classified as skewed when 80% or more of the cells preferentially inactivated the same X chromosome.
Main Outcome Measures: We assessed the prevalence of skewed XCI.
Results: The frequency of skewed XCI in female twins with AITD, GD, and HT was 34, 37, and 31%, respectively, which was higher than the prevalence in the corresponding control populations, 11% (P = 0.003), 14% (P = 0.045), and 8% (P = 0.057), respectively. Similar results were found in twin pairs discordant for AITD. Overall, skewed XCI was associated with an increased risk of developing AITD, with an odds ratio of 9.0 (95% confidence interval, 1.64–49.4) (P = 0.022).
Conclusion: These observations suggest a possible role of XCI in the etiology of AITD and may in part explain the female preponderance of AITD.
Oxford University Press