Cell-based biosensors (CBBs) are becoming important tools for biosecurity applications and
rapid diagnostics in food microbiology for their unique capability of detecting physiologically
hazardous materials. A multi-well plate-based biosensor containing B-cell hybridoma, Ped-
2E9, encapsulated in type I collagen matrix, was developed for rapid detection of viable cells
of pathogenic Listeria, the toxin listeriolysin O, and the enterotoxin from Bacillus species.
This sensor measures the alkaline phosphatase release from infected Ped-2E9 cells …