[HTML][HTML] Enhancement of guanine-nucleotide exchange activity of C3G for Rap1 by the expression of Crk, CrkL, and Grb2
T Ichiba, Y Kuraishi, O Sakai, S Nagata… - Journal of Biological …, 1997 - ASBMB
Crk is an adaptor protein that consists almost entirely of SH2 and SH3 domains. We have
previously demonstrated, by using in vivo and in vitro systems, that C3G, which was
identified as a Crk SH3 domain-binding guanine nucleotide exchange factor, specifically
activates Rap1. C3G also binds to other adaptor proteins, including CrkL and Grb2. In the
present study, we analyzed the effect of Crk, CrkL, and Grb2 on the C3G-Rap1 pathway.
Expression of Crk, CrkL, and Grb2 with C3G in Cos1 cells significantly increased the ratio of …
previously demonstrated, by using in vivo and in vitro systems, that C3G, which was
identified as a Crk SH3 domain-binding guanine nucleotide exchange factor, specifically
activates Rap1. C3G also binds to other adaptor proteins, including CrkL and Grb2. In the
present study, we analyzed the effect of Crk, CrkL, and Grb2 on the C3G-Rap1 pathway.
Expression of Crk, CrkL, and Grb2 with C3G in Cos1 cells significantly increased the ratio of …