[HTML][HTML] FCP1 phosphorylation by casein kinase 2 enhances binding to TFIIF and RNA polymerase II carboxyl-terminal domain phosphatase activity
B Palancade, MF Dubois, O Bensaude - Journal of Biological Chemistry, 2002 - ASBMB
Dephosphorylation of RNA polymerase II carboxyl-terminal domain (CTD) is required to
resume sequential transcription cycles. FCP1 (TFIIF-dependent CTD phosphatase 1) is the
only known phosphatase targeting RNAP II CTD. Here we show that in Xenopus laevis cells,
xFCP1 is a phosphoprotein. On the basis of biochemical fractionation and drug sensitivity,
casein kinase 2 (CK2) is shown to be the major kinase involved in xFCP1 phosphorylation in
X. laevis egg extracts. CK2 phosphorylates xFCP1 mainly at a cluster of serines centered on …
resume sequential transcription cycles. FCP1 (TFIIF-dependent CTD phosphatase 1) is the
only known phosphatase targeting RNAP II CTD. Here we show that in Xenopus laevis cells,
xFCP1 is a phosphoprotein. On the basis of biochemical fractionation and drug sensitivity,
casein kinase 2 (CK2) is shown to be the major kinase involved in xFCP1 phosphorylation in
X. laevis egg extracts. CK2 phosphorylates xFCP1 mainly at a cluster of serines centered on …