Association of allergen-specific regulatory T cells with the onset of clinical tolerance to milk protein
WG Shreffler, N Wanich, M Moloney… - Journal of Allergy and …, 2009 - Elsevier
BACKGROUND: About 70% of children with milk allergy tolerate extensively heated milk
(HM) products and outgrow their allergy earlier than those who react to HM. OBJECTIVE: To
test the hypothesis that HM-tolerant children have a higher precursor frequency of adaptive
allergen-specific regulatory T (Treg) cells. METHODS: Allergic, HM-tolerant, outgrown, or
control subjects were defined by oral food challenge. PBMCs were cultured with purified
caseins and controls for 7 days, and proliferating CD25+ CD27+ Treg cells were identified …
(HM) products and outgrow their allergy earlier than those who react to HM. OBJECTIVE: To
test the hypothesis that HM-tolerant children have a higher precursor frequency of adaptive
allergen-specific regulatory T (Treg) cells. METHODS: Allergic, HM-tolerant, outgrown, or
control subjects were defined by oral food challenge. PBMCs were cultured with purified
caseins and controls for 7 days, and proliferating CD25+ CD27+ Treg cells were identified …