The species of acyl-CoA in subcellular fractions of type II cells isolated from adult rat lung and their incorporation into phosphatidic acid
JN Den Breejen, JJ Batenburg… - Biochimica et Biophysica …, 1989 - Elsevier
Microsomes and cytosol were prepared from type II cells isolated from adult rat lung. Upon
determination of the acyl-CoA composition in the microsomes, we found 49% palmitoyl-CoA,
2% myristoyl-CoA, 21% stearoyl-CoA, 5% palmitoleoyl-CoA, 16% oleoyl-CoA, 5% linoleoyl-
CoA and 2% arachidonoyl-CoA. The acyl-CoA composition of the cytosol was very similar.
Upon incubation of type II cell microsomes with [U-14 C] glycerol 3-phosphate and with acyl-
CoA species mixed in the proportions in which they were found in this cell fraction, approx …
determination of the acyl-CoA composition in the microsomes, we found 49% palmitoyl-CoA,
2% myristoyl-CoA, 21% stearoyl-CoA, 5% palmitoleoyl-CoA, 16% oleoyl-CoA, 5% linoleoyl-
CoA and 2% arachidonoyl-CoA. The acyl-CoA composition of the cytosol was very similar.
Upon incubation of type II cell microsomes with [U-14 C] glycerol 3-phosphate and with acyl-
CoA species mixed in the proportions in which they were found in this cell fraction, approx …
