ELISA procedures for the measurement of IgG subclass antibodies to bacterial antigens

S Ruths, PC Driedijk, RS Weening, TA Out - Journal of immunological …, 1991 - Elsevier
We have developed enzyme-linked immunosorbent assays (ELISA) of IgG subclass
antibodies against whole bacteria and bacterial antigens using enzyme-labelled mouse
monoclonal antibodies. The properties of different anti-subclass antibodies were compared.
In sera from 18 healthy adults we measured the IgG subclass distribution of specific
antibodies against Staphylococcus aureus and Haemophilus influenzae b and against
distinct bacterial components: pneumococcal capsular polysaccharides, dextran and tetanus …