Direct interaction of the mitochondrial membrane protein carnitine palmitoyltransferase I with Bcl-2

MB Paumen, Y Ishida, H Han, M Muramatsu… - Biochemical and …, 1997 - Elsevier
MB Paumen, Y Ishida, H Han, M Muramatsu, Y Eguchi, Y Tsujimoto, T Honjo
Biochemical and biophysical research communications, 1997Elsevier
To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2
the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2.
Direct protein interaction was confirmed in a GST binding assay and in co-
immunoprecipitations using two different kinds of anti-Bcl-2 antibodies.
To demonstrate interaction between the mitochondrial membrane proteins CPT I and Bcl-2 the yeast two-hybrid system was used. Full-length CPT I was required for binding to Bcl-2. Direct protein interaction was confirmed in a GST binding assay and in co-immunoprecipitations using two different kinds of anti-Bcl-2 antibodies.
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