[HTML][HTML] Expression, selection, and organellar targeting of the green fluorescent protein in Toxoplasma gondii
We have engineered a mutant version of the green fluorescent protein GFP (Cormack et al.
Selected for bright fluorescence in E. coli. Gene 1996; 173: 33–38) for expression in the
protozoan parasite Toxoplasma gondii. Although intact GFP was not expressed at any
detectable level, GFP fusion proteins could be detected by fluorescence microscopy, flow
cytometry (FACS), and immunoblotting. Both extracellular tachyzoites and T. gondii-infected
host cells could readily be sorted by FACS, which should facilitate a variety of selection …
Selected for bright fluorescence in E. coli. Gene 1996; 173: 33–38) for expression in the
protozoan parasite Toxoplasma gondii. Although intact GFP was not expressed at any
detectable level, GFP fusion proteins could be detected by fluorescence microscopy, flow
cytometry (FACS), and immunoblotting. Both extracellular tachyzoites and T. gondii-infected
host cells could readily be sorted by FACS, which should facilitate a variety of selection …
