Abelson murine leukaemia virus protein is phosphorylated in vitro to form phosphotyrosine
ON Witte, A Dasgupta, D Baltimore - Nature, 1980 - nature.com
ON Witte, A Dasgupta, D Baltimore
Nature, 1980•nature.comThe Abelson murine leukaemia virus protein (P120) can become phosphorylated in vitro by
[γ-32P] ATP. The protein has been purified from cell membranes to the point that in specific
conditions virtually all of the incorporated 32P is in P120. The reaction is stimulated by Mn2+
and Mg2+ but not Ca2+ and is very rapid even at 0° C. The phosphate is linked to P120 at
tyrosine, a linkage not previously reported for a phosphorylation reaction. Phosphorylation
may be involved in the transforming activity of viruses that cause leukaemia as well as …
[γ-32P] ATP. The protein has been purified from cell membranes to the point that in specific
conditions virtually all of the incorporated 32P is in P120. The reaction is stimulated by Mn2+
and Mg2+ but not Ca2+ and is very rapid even at 0° C. The phosphate is linked to P120 at
tyrosine, a linkage not previously reported for a phosphorylation reaction. Phosphorylation
may be involved in the transforming activity of viruses that cause leukaemia as well as …
Abstract
The Abelson murine leukaemia virus protein (P120) can become phosphorylated in vitro by [γ-32P]ATP. The protein has been purified from cell membranes to the point that in specific conditions virtually all of the incorporated 32P is in P120. The reaction is stimulated by Mn2+ and Mg2+ but not Ca2+ and is very rapid even at 0 °C. The phosphate is linked to P120 at tyrosine, a linkage not previously reported for a phosphorylation reaction. Phosphorylation may be involved in the transforming activity of viruses that cause leukaemia as well as sarcomas.
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