We recently have reported the immunolocalization of epidermal growth factor (EGF) in
mouse kidney by light microscopy. In the present study, we have investigated the
ultrastructural localization of EGF by the preembedding immunoperoxidase method for
electron microscopy. EGF immunoreactivity was present on the apical plasma membrane of
the cells of the thick ascending limb of Henle and the distal convoluted tubule. The macula
densa, by contrast, lacked immunoreactivity. Based on functional evidence, a possible …