[HTML][HTML] Specificity and promiscuity in phosphoinositide binding by pleckstrin homology domains
Pleckstrin homology (PH) domains are small protein modules involved in recruitment of
signaling molecules to cellular membranes, in some cases by binding specific
phosphoinositides. We describe use of a convenient" dot-blot" approach to screen 10
different PH domains for those that recognize particular phosphoinositides. Each PH domain
bound phosphoinositides in the assay, but only two (from phospholipase C-δ 1 and Grp1)
showed clear specificity for a single species. Using soluble inositol phosphates, we show …
signaling molecules to cellular membranes, in some cases by binding specific
phosphoinositides. We describe use of a convenient" dot-blot" approach to screen 10
different PH domains for those that recognize particular phosphoinositides. Each PH domain
bound phosphoinositides in the assay, but only two (from phospholipase C-δ 1 and Grp1)
showed clear specificity for a single species. Using soluble inositol phosphates, we show …
