A ras-related gene from the lower eukaryote Dictyostelium that is highly conserved relative to the human rap genes
SM Robbins, VV Suttorp, G Weeks… - Nucleic acids …, 1990 - academic.oup.com
SM Robbins, VV Suttorp, G Weeks, GB Spiegelman
Nucleic acids research, 1990•academic.oup.comThe cellular slime mold Dictyostelium discoideum contains two ras genes, Dd rasG and Dd
ras that are differentially expressed during development. We have characterized a gene that
hybridized to both Dd ras and Dd ras G under low, but not under high stringency conditions.
The deduced amino acid sequence is highly conserved with respect to the human rap (K rev-
1, smg 21) proteins and the corresponding gene has been designated Dd rap 1. The Dd rap
1 gene is expressed at all stages during development but is expressed maximally during the …
ras that are differentially expressed during development. We have characterized a gene that
hybridized to both Dd ras and Dd ras G under low, but not under high stringency conditions.
The deduced amino acid sequence is highly conserved with respect to the human rap (K rev-
1, smg 21) proteins and the corresponding gene has been designated Dd rap 1. The Dd rap
1 gene is expressed at all stages during development but is expressed maximally during the …
Abstract
The cellular slime mold Dictyostelium discoideum contains two ras genes, DdrasG and Ddras that are differentially expressed during development. We have characterized a gene that hybridized to both Ddras and DdrasG under low, but not under high stringency conditions. The deduced amino acid sequence is highly conserved with respect to the human rap (Krev-1, smg21) proteins and the corresponding gene has been designated Ddrap1. The Ddrap1 gene is expressed at all stages during development but is expressed maximally during the aggregation and culmination periods when the expression of Ddras and DdrasG is declining. During vegetative growth and early development Ddrap1 cDNA hybridizes to a single mRNA of 1.1 kb. As development progresses the level of this mRNA declines and messages of 1.0 and 1.3 kb appear.
Oxford University Press