Isolation and characterization of the gene encoding phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae
MD Valdés-Hevia, R de la Guerra, C Gancedo - FEBS letters, 1989 - Elsevier
MD Valdés-Hevia, R de la Guerra, C Gancedo
FEBS letters, 1989•ElsevierThe yeast PCK1 gene coding for phosphoenolpyruvate carboxykinase (PEPCK) was
isolated by functional complementation of pck1 strains from S. cerevisiae. Only one copy of
the gene was found per haploid yeast genome. An RNA of about 2 kb which hybridized with
a DNA probe internal to the PCK1 gene was found only in cells growing in non-fermentable
carbon sources. Yeast strains carrying multiple copies of the PCK1 gene showed normal
catabolite repression of PEPCK except those carrying the shortest insertion complementing …
isolated by functional complementation of pck1 strains from S. cerevisiae. Only one copy of
the gene was found per haploid yeast genome. An RNA of about 2 kb which hybridized with
a DNA probe internal to the PCK1 gene was found only in cells growing in non-fermentable
carbon sources. Yeast strains carrying multiple copies of the PCK1 gene showed normal
catabolite repression of PEPCK except those carrying the shortest insertion complementing …
Abstract
The yeast PCK1 gene coding for phosphoenolpyruvate carboxykinase (PEPCK) was isolated by functional complementation of pck1 strains from S. cerevisiae. Only one copy of the gene was found per haploid yeast genome. An RNA of about 2 kb which hybridized with a DNA probe internal to the PCK1 gene was found only in cells growing in non-fermentable carbon sources. Yeast strains carrying multiple copies of the PCK1 gene showed normal catabolite repression of PEPCK except those carrying the shortest insertion complementing the mutation (2.2 kb) that presented an altered kinetics of derepression. Catabolite inactivation was decreased in strains transformed with multicopy plasmids carrying the PCK1 gene.
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