[HTML][HTML] The N-terminal anchor sequences of 11β-hydroxysteroid dehydrogenases determine their orientation in the endoplasmic reticulum membrane
A Odermatt, P Arnold, A Stauffer, BM Frey… - Journal of Biological …, 1999 - ASBMB
11β-Hydroxysteroid dehydrogenase enzymes (11β-HSD) regulate the ratio of active
endogenous glucocorticoids to their inactive keto-metabolites, thereby controlling the access
of glucocorticoids to their cognate receptors. In this study, the topology and intracellular
localization of 11β-HSD1 and 11β-HSD2 have been analyzed by immunohistochemistry and
protease protection assays of in vitro transcription/translation products. 11β-HSD constructs,
tagged with the FLAG epitope, were transiently expressed in HEK-293 cells. The enzymatic …
endogenous glucocorticoids to their inactive keto-metabolites, thereby controlling the access
of glucocorticoids to their cognate receptors. In this study, the topology and intracellular
localization of 11β-HSD1 and 11β-HSD2 have been analyzed by immunohistochemistry and
protease protection assays of in vitro transcription/translation products. 11β-HSD constructs,
tagged with the FLAG epitope, were transiently expressed in HEK-293 cells. The enzymatic …