[HTML][HTML] In vivo footprinting of the human 11β-hydroxysteroid dehydrogenase type 2 promoter: Evidence for cell-specific regulation by Sp1 and Sp3
AR Nawrocki, CE Goldring, RM Kostadinova… - Journal of Biological …, 2002 - ASBMB
11β-Hydroxysteroid dehydrogenase type 2 is selectively expressed in aldosterone target
tissues, where it confers aldosterone selectivity for the mineralocorticoid receptor by
inactivating 11β-hydroxyglucocorticoids with a high affinity for the mineralocorticoid receptor.
The present investigation aimed to elucidate the mechanisms accounting for the rigorous
control of theHSD11B2 gene in humans. Using dimethyl sulfatein vivo footprinting via
ligation-mediated PCR, we identified potentially important regions for HSD11B2regulation in …
tissues, where it confers aldosterone selectivity for the mineralocorticoid receptor by
inactivating 11β-hydroxyglucocorticoids with a high affinity for the mineralocorticoid receptor.
The present investigation aimed to elucidate the mechanisms accounting for the rigorous
control of theHSD11B2 gene in humans. Using dimethyl sulfatein vivo footprinting via
ligation-mediated PCR, we identified potentially important regions for HSD11B2regulation in …