Differential effects of eicosapentaenoic acid on glycerolipid and apolipoprotein B metabolism in primary human hepatocytes compared to HepG2 cells and primary rat …
Y Lin, MJ Smit, R Havinga, HJ Verkade, RJ Vonk… - … et Biophysica Acta (BBA …, 1995 - Elsevier
We compared the effects of eicosapentaenoic acid (EPA) and oleic acid (OA) on glycerolipid
and apolipoprotein B (apoB) metabolism in primary human hepatocytes, HepG2 cells and
primary rat hepatocytes. Cells were incubated for 1 to 5 h with 0.25 mM bovine serum
albumin in the absence (control) or presence of 1 mM of EPA or OA. Synthesis and secretion
of [3H] glycerolipid were determined after 1 h incubation with [3H] glycerol. Cellular and
medium apoB abundance was semi-quantitatively estimated in human cells by Western …
and apolipoprotein B (apoB) metabolism in primary human hepatocytes, HepG2 cells and
primary rat hepatocytes. Cells were incubated for 1 to 5 h with 0.25 mM bovine serum
albumin in the absence (control) or presence of 1 mM of EPA or OA. Synthesis and secretion
of [3H] glycerolipid were determined after 1 h incubation with [3H] glycerol. Cellular and
medium apoB abundance was semi-quantitatively estimated in human cells by Western …
