[HTML][HTML] Molecular cloning, functional characterization and mRNA expression analysis of the murine chemokine receptor CCR6 and its specific ligand MIP-3α
R Varona, A Zaballos, J Gutiérrez, P Martı́n, F Roncal… - FEBS letters, 1998 - Elsevier
FEBS letters, 1998•Elsevier
We have cloned the murine CCR6 receptor and its ligand, the β-chemokine mMIP-3α.
Calcium mobilization assays performed with mCCR6 transfectants showed significant
responses upon addition of mMIP-3α. Murine MIP-3α RNA is expressed in thymus, small
intestine and colon, whereas mCCR6 RNA is expressed in spleen and lymph nodes. RT-
PCR analysis of FACS-sorted lymphoid and antigen presenting cell subsets showed
mCCR6 expression mainly in B cells, CD8− splenic dendritic cells and CD4+ T cells. The …
Calcium mobilization assays performed with mCCR6 transfectants showed significant
responses upon addition of mMIP-3α. Murine MIP-3α RNA is expressed in thymus, small
intestine and colon, whereas mCCR6 RNA is expressed in spleen and lymph nodes. RT-
PCR analysis of FACS-sorted lymphoid and antigen presenting cell subsets showed
mCCR6 expression mainly in B cells, CD8− splenic dendritic cells and CD4+ T cells. The …
We have cloned the murine CCR6 receptor and its ligand, the β-chemokine mMIP-3α. Calcium mobilization assays performed with mCCR6 transfectants showed significant responses upon addition of mMIP-3α. Murine MIP-3α RNA is expressed in thymus, small intestine and colon, whereas mCCR6 RNA is expressed in spleen and lymph nodes. RT-PCR analysis of FACS-sorted lymphoid and antigen presenting cell subsets showed mCCR6 expression mainly in B cells, CD8− splenic dendritic cells and CD4+ T cells. The cloning and functional characterization of the mCCR6 and mMIP-3α will allow the study of the role of these proteins in mouse models of inflammation and immunity.
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