[HTML][HTML] Oxidative stress in cell culture: an under-appreciated problem?
B Halliwell - FEBS letters, 2003 - Elsevier
FEBS letters, 2003•Elsevier
Cell culture studies have given much valuable information about mechanisms of metabolism
and signal transduction and of regulation of gene expression, proliferation, senescence, and
death. However, cells in culture may behave differently from cells in vivo in many ways. One
of these is that cell culture imposes a state of oxidative stress on cells. I argue that cells that
survive and grow in culture might use ROS-dependent signal transduction pathways that
rarely or never operate in vivo. A further problem is that cell culture media can catalyse the …
and signal transduction and of regulation of gene expression, proliferation, senescence, and
death. However, cells in culture may behave differently from cells in vivo in many ways. One
of these is that cell culture imposes a state of oxidative stress on cells. I argue that cells that
survive and grow in culture might use ROS-dependent signal transduction pathways that
rarely or never operate in vivo. A further problem is that cell culture media can catalyse the …
Cell culture studies have given much valuable information about mechanisms of metabolism and signal transduction and of regulation of gene expression, proliferation, senescence, and death. However, cells in culture may behave differently from cells in vivo in many ways. One of these is that cell culture imposes a state of oxidative stress on cells. I argue that cells that survive and grow in culture might use ROS-dependent signal transduction pathways that rarely or never operate in vivo. A further problem is that cell culture media can catalyse the oxidation of compounds added to them, resulting in apparent cellular effects that are in fact due to oxidation products such as ROS. Such artefacts may have affected many studies on the effects of ascorbate, thiols, flavonoids and other polyphenolic compounds on cells in culture.
Elsevier
