[HTML][HTML] Persistent corneal haze after excimer laser photokeratectomy in plasminogen-deficient mice
AF Drew, HL Schiman, KW Kombrinck… - … & visual science, 2000 - arvojournals.org
AF Drew, HL Schiman, KW Kombrinck, TH Bugge, JL Degen, AH Kaufman
Investigative ophthalmology & visual science, 2000•arvojournals.orgpurpose. Excimer laser photorefractive keratectomy creates a nonvascular wound of the
cornea. Fibrin deposition and resolution after excimer laser photokeratectomy were
investigated in relation to corneal repair and restoration of clarity in mice with a genetic
deficiency of plasminogen. methods. A Summit Apex Laser (Summit, Waltham, MA) was
used to perform 2-mm, 175-pulse, transepithelial photoablations that resulted in deep
stromal keratectomies. Photokeratectomy was performed on the corneas of plasminogen …
cornea. Fibrin deposition and resolution after excimer laser photokeratectomy were
investigated in relation to corneal repair and restoration of clarity in mice with a genetic
deficiency of plasminogen. methods. A Summit Apex Laser (Summit, Waltham, MA) was
used to perform 2-mm, 175-pulse, transepithelial photoablations that resulted in deep
stromal keratectomies. Photokeratectomy was performed on the corneas of plasminogen …
Abstract
purpose. Excimer laser photorefractive keratectomy creates a nonvascular wound of the cornea. Fibrin deposition and resolution after excimer laser photokeratectomy were investigated in relation to corneal repair and restoration of clarity in mice with a genetic deficiency of plasminogen.
methods. A Summit Apex Laser (Summit, Waltham, MA) was used to perform 2-mm, 175-pulse, transepithelial photoablations that resulted in deep stromal keratectomies. Photokeratectomy was performed on the corneas of plasminogen-deficient (Plg−/−) mice and littermate control animals. Eyes were examined for re-epithelialization and clarity throughout the 21-day observational period. Histologic sections were taken during the observational period and fibrin (ogen) was detected immunohistochemically.
results. Re-epithelialization was rapid and complete within 3 days in both control andPlg−/− animals. Exuberant corneal fibrin (ogen) deposition was noted in Plg−/− mice and sparse fibrin (ogen) deposition in control mice on days 1 and 3 after injury. Fibrin (ogen) deposits resolved in control mice but persisted in Plg−/− mice (74% of eyes at 21 days; P< 0.004). Corneal opacification, scarring, and the presence of anterior chamber fibrin (ogen) occurred in plasminogen-deficient mice but not in control mice.
conclusions. Fibrin (ogen) deposition occurs during corneal wound repair after photokeratectomy. Impaired fibrinolysis in Plg−/− mice caused persistent stromal fibrin deposits that correlated with the development of corneal opacity.
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