[HTML][HTML] Mitochondrial membrane permeabilization and superoxide production during apoptosis: a single-cell analysis
H Düssmann, D Kögel, M Rehm… - Journal of Biological …, 2003 - ASBMB
The temporal relationship between mitochondrial membrane permeabilization and reactive
oxygen species production during apoptosis remains unknown. We analyzed the rate of
superoxide production of human breast carcinoma cells expressing a cytochromec-green
fluorescent protein (cyt-c-GFP) fusion protein at the single-cell level during apoptosis. In
cells treated with the proapoptotic agents staurosporine (3 μm) or tumor necrosis factor-α
(100 ng/ml), the release of cyt-c-GFP was individually set for each cell, and the majority of …
oxygen species production during apoptosis remains unknown. We analyzed the rate of
superoxide production of human breast carcinoma cells expressing a cytochromec-green
fluorescent protein (cyt-c-GFP) fusion protein at the single-cell level during apoptosis. In
cells treated with the proapoptotic agents staurosporine (3 μm) or tumor necrosis factor-α
(100 ng/ml), the release of cyt-c-GFP was individually set for each cell, and the majority of …