[HTML][HTML] Visualizing superoxide production in normal and diabetic rat islets of Langerhans
VP Bindokas, A Kuznetsov, S Sreenan… - Journal of Biological …, 2003 - ASBMB
Oxygen free radicals have been implicated in β-cell dysfunction and apoptosis associated
with type 1 and type 2 diabetes mellitus. The roles of free radicals in diabetes have thus far
been defined indirectly by monitoring oxidative tissue damage and the effects of
antioxidants, free radical scavengers, and overexpression of superoxide dismutase. We
employed the superoxide-mediated oxidation of hydroethidine to ethidium to dynamically
and directly assess the relative rates of mitochondrial superoxide anion generation in …
with type 1 and type 2 diabetes mellitus. The roles of free radicals in diabetes have thus far
been defined indirectly by monitoring oxidative tissue damage and the effects of
antioxidants, free radical scavengers, and overexpression of superoxide dismutase. We
employed the superoxide-mediated oxidation of hydroethidine to ethidium to dynamically
and directly assess the relative rates of mitochondrial superoxide anion generation in …