A reliability test of standard-based quantitative PCR: exogenous vs endogenous standards
LD Ke, Z Chen, WKA Yung - Molecular and cellular probes, 2000 - Elsevier
The quantitative measurement of gene expression requires consistent and reliable
standards. At least two categories of standards, endogenous and exogenous, are currently
used for quantitative PCR. The reliability of these two methods, however, has not been
carefully compared. We hypothesized that a reliable quantitative PCR assay would be able
to detect known dilutions of a given single-stranded (ss-) cDNA. By measuring VEGF ss-
cDNA copy numbers or signal ratios of GAPDH/VEGF in 10× and 100× diluted samples of …
standards. At least two categories of standards, endogenous and exogenous, are currently
used for quantitative PCR. The reliability of these two methods, however, has not been
carefully compared. We hypothesized that a reliable quantitative PCR assay would be able
to detect known dilutions of a given single-stranded (ss-) cDNA. By measuring VEGF ss-
cDNA copy numbers or signal ratios of GAPDH/VEGF in 10× and 100× diluted samples of …