[HTML][HTML] Promoter-specific modulation of insulin-like growth factor II genomic imprinting by inhibitors of DNA methylation
JF Hu, TH Vu, AR Hoffman - Journal of Biological Chemistry, 1996 - ASBMB
The insulin-like growth factor II (IGF-II) gene is maternally imprinted in most normal tissues
with only the paternal allele being transcribed. In several human tumors, however, IGF-II is
expressed from both parental alleles. To explore the underlying mechanism of IGF-II
imprinting, we have examined the effect of DNA demethylation in cultured human and
mouse astrocyte cells. An increased expression of IGF-II was observed when these cells
were treated with the DNA demethylating agents, 5-azacytidine or 2-deoxy-5-azacytidine …
with only the paternal allele being transcribed. In several human tumors, however, IGF-II is
expressed from both parental alleles. To explore the underlying mechanism of IGF-II
imprinting, we have examined the effect of DNA demethylation in cultured human and
mouse astrocyte cells. An increased expression of IGF-II was observed when these cells
were treated with the DNA demethylating agents, 5-azacytidine or 2-deoxy-5-azacytidine …