[HTML][HTML] Growth factor protection against cytokine-induced apoptosis in neonatal rat islets of Langerhans: role of Fas
M Harrison, AM Dunger, S Berg, J Mabley, N John… - FEBS letters, 1998 - Elsevier
M Harrison, AM Dunger, S Berg, J Mabley, N John, MHL Green, IC Green
FEBS letters, 1998•ElsevierTreatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1β 10−
10 M, tumour necrosis factor-α 10− 10 M, interferon-γ 5 U/ml) led to extensive cell death,
which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-
treatment with insulin (25 ng/ml) or insulin-like growth factor-1 (10− 8 M) gave only partial
protection against cell killing, but prevented the Fas-mediated component. In the absence of
cytokine treatment, Fas-mediated killing was not observed.
10 M, tumour necrosis factor-α 10− 10 M, interferon-γ 5 U/ml) led to extensive cell death,
which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-
treatment with insulin (25 ng/ml) or insulin-like growth factor-1 (10− 8 M) gave only partial
protection against cell killing, but prevented the Fas-mediated component. In the absence of
cytokine treatment, Fas-mediated killing was not observed.
Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1β 10−10 M, tumour necrosis factor-α 10−10 M, interferon-γ 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or insulin-like growth factor-1 (10−8 M) gave only partial protection against cell killing, but prevented the Fas-mediated component. In the absence of cytokine treatment, Fas-mediated killing was not observed.
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