Tetranitromethane. A Reagent for the Nitration of Tyrosyl Residues in Proteins*
M Sokolovsky, JF Riordan, BL Vallee - Biochemistry, 1966 - ACS Publications
M Sokolovsky, JF Riordan, BL Vallee
Biochemistry, 1966•ACS PublicationsTetranitromethane (TNM) has been found to be a specific, mild reagent for the nitration of
tyrosyl residues of proteins at pH 8. Oxidation of cysteine, the only other amino acidaffected,
can be differentiated by carrying out the reaction at pH 6 where tyrosine is not altered. By
chromatography 3-nitrotyrosine has been identified as the product of the reaction of TNM
with tyrosyl-containing peptides and proteins, resulting in an absorption band with a
maximum at
tyrosyl residues of proteins at pH 8. Oxidation of cysteine, the only other amino acidaffected,
can be differentiated by carrying out the reaction at pH 6 where tyrosine is not altered. By
chromatography 3-nitrotyrosine has been identified as the product of the reaction of TNM
with tyrosyl-containing peptides and proteins, resulting in an absorption band with a
maximum at
Tetranitromethane (TNM) has been found to be a specific, mild reagent for the nitration of tyrosyl residues of proteins at pH 8. Oxidation of cysteine, the only other amino acidaffected, can be differentiated by carrying out the reaction at pH 6 where tyrosine is not altered. By chromatography 3-nitrotyrosine has been identified as the product of the reaction of TNM with tyrosyl-containing peptides and proteins, resulting in an absorption band with a maximum at
ACS Publications