[HTML][HTML] Mutant of insulin receptor substrate-1 incapable of activating phosphatidylinositol 3-kinase did not mediate insulin-stimulated maturation of Xenopus laevis …
R Yamamoto-Honda, Z Honda, K Ueki, K Tobe… - Journal of Biological …, 1996 - ASBMB
Insulin receptor substrate-1 (IRS-1) is rapidly phosphorylated on multiple tyrosine residues
in response to insulin and binds several Src homology 2 domain-containing proteins,
thereby initiating downstream signaling. To assess the tyrosine phosphorylation sites that
mediate relevant downstream signaling and biological effects, we created site-directed
mutants of IRS-1 and overexpressed them in the Xenopus laevis oocyte. In oocytes
overexpressing IRS-1 or IRS-1-895F (Tyr-895 replaced with phenylalanine), insulin …
in response to insulin and binds several Src homology 2 domain-containing proteins,
thereby initiating downstream signaling. To assess the tyrosine phosphorylation sites that
mediate relevant downstream signaling and biological effects, we created site-directed
mutants of IRS-1 and overexpressed them in the Xenopus laevis oocyte. In oocytes
overexpressing IRS-1 or IRS-1-895F (Tyr-895 replaced with phenylalanine), insulin …
